Class time and location

Wednesdays 2:00-3:00 (Room: SCI-S Room 179)

* No self-evaluation

* No peer-evaluation

* No response to peer evaluation

* Absence

* No presentation

Participants:

1. Sara Alsrayea./
2. Raghad Alrefaei./
3. Ali Garashi./

Seminar Topics: Medical Biotechnology.

HA selected examples:

• Koch et al., Skin Tissue Generation by Laser Cell Printing.

• Reiffel et al., High-Fidelity Tissue Engineering of Patient-Specific Auricles for Reconstruction of Pediatric Microtia and Other Auricular Deformities

• Lee et al., Design and fabrication of human skin by three-dimensional bioprinting

Your presentation should include:

1. Title, student name, course, presentation date-slide.
2. Paper front page and abstract-slide.
3. General info about the organism-slide(s).
4. General info about the problem/goal of the study-slide(s).
5. General description about the methods used-silde(s).
6. Result (each figure or table in a separate slide).
7. Discussion slide about the usefulness of the study.
8. A final slide about your ideas/suggestions/ethical issues for implementing such studies in Kuwait.

To Do:

1.  Search for a biotechnology research paper that is related to the seminar topic and send a pdf to your instructor for approval.
2. Make a powerpoint file named (YourName_501).
3. Send a copy of your presentation a day before your actual presentation.
4. Non-presenters should read the paper and prepare to engage in discussion.
5. If it is possible, we will repeat the process with a second publication.

Useful files:

1. Powerpoint presentation template ().
2. Self evaluation template ().
3. Peer evaluation template ().
4. Response of peer evaluation template ().

Topics:

Seminar 1 – 5/10/22: Course planning, scientific publications, finding good ones

– HA

Seminar 2 – 12/10/22: How to read a scientific paper? – HA

Seminar 3 – 19/10/22: Making a good presentation – HA

Seminar 4 – 26/10/22: Hematopoietic Stem Cell Gene Therapy with a Lentiviral Vector in X-Linked Adrenoleukodystrophy – Sara Alsrayea

Seminar 5 – 2/11/22: Development of small interfering RNA delivery system using PEI-PEG-APRPG polymer for antiangiogenic vascular endothelial growth factor tumor-targeted therapy – Raghad Alrefaei

Seminar 10 – 7/12/22: Studies on the immunogenic potential of plant-expressed cholera toxin B subunit – Sara Alsrayea

Seminar 11 – 14/12/22: Tumor selective uptake of drug-nanodiamond complexes improves therapeutic outcome in pancreatic cancer – Raghad Alrefaei 

* No self-evaluation

* No peer-evaluation

* No response to peer evaluation

* No camera

* Absence

* No presentation

Participants:

1. Maryam Alshiha – MS./
2. Afnan Mohamed – AM.*/
3. Mayar Albdawi – MB./
4. Safeyah Alqabandi – SQ.*/
5. Ahmad Alfoudari – AA.*/
6. Zainab Mohammad – ZM./
7. Abdulwahab Aldousari – AD./

Seminar Topics: Biotechnology of Animal and plant products.

HA selected examples:

• Douglas V. Shaw & Kirk D. Larson (2008) Performance of early-generation and modern strawberry cultivars from the University of California breeding programme in growing systems simulating traditional and modern horticulture, The Journal of Horticultural Science and Biotechnology, 83:5, 648-652
• Shaw et al. (2006). Genotypic Variation for Resistance to Phytophthora cactorum in a California Strawberry Breeding Population, Journal of the American Society for Horticultural Science, 131(5), 687-690
• Edger et al. (2019). Origin and evolution of the octoploid strawberry genome. Nat Genet 51, 541–547 
• Castillejo et al. (2020). Allelic Variation of MYB10 Is the Major Force Controlling Natural Variation in Skin and Flesh Color in Strawberry (Fragaria spp.) Fruit. The Plant Cell,  32(12): 3723–3749

Your presentation should include:

1. Title, student name, course, presentation date-slide.
2. Paper front page and abstract-slide.
3. General info about the organism-slide(s).
4. General info about the problem/goal of the study-slide(s).
5. General description about the methods used-silde(s).
6. Result (each figure or table in a separate slide).
7. Discussion slide about the usefulness of the study.
8. A final slide about your ideas/suggestions/ethical issues for implementing such studies in Kuwait.

To Do:

1.  Search for a biotechnology research paper that is related to the seminar topic and send a pdf to your instructor for approval.
2. Make a powerpoint file named (YourName_501).
3. Send a copy of your presentation a day before your actual presentation.
4. Non-presenters should read the paper and prepare to engage in discussion.
5. If it is possible, we will repeat the process with a second publication.

Useful files:

1. Powerpoint presentation template ().
2. Self evaluation template ().
3. Peer evaluation template ().
4. Response of peer evaluation template ().

Topics:

Seminar 1 – 26/10/21: Course planning, scientific publications, finding good ones

– HA

Seminar 2 – 02/11/21: How to read a scientific paper? – HA

Seminar 3 – 09/11/21: Making a good presentation – HA

Seminar 4 – 16/11/21: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Safron (Crocus sativus L.) – MS (Plant production), Presentation: MaryamAlshiha_501

Seminar 5 – 23/11/21: Generation of microalga Chlamydomonas reinhardtii expressing shrimp antiviral dsRNA without supplementation of antibiotics – AM (Animal production), Presentation: AfnanKhaled_510

Seminar 6 – 30/11/21: Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1promoter in citrus – MB (Plant production), Presentation: MayarAlbadawi_510

Seminar 7 – 07/12/21: Production of artificial synthetic spidroin gene 4S-transgenic cloned sheep embryos using somatic cell nuclear transfer – SQ (Animal production), Presentation:

Seminar 8 – 14/12/21: Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif – AA (Plant production), Presentation:

Seminar 9 – 21/12/21: The application of bioflocs technology to protect brine shrimp (Artemia franciscana) from pathogenic Vibrio harveyi – ZM (Animal production), Presentation:

Seminar 10 – 28/12/21: Production of bioplastic from potato peel waste and investigation of its biodegradability – AD (Plant production), Presentation:

Seminar 11 – 04/01/22: In vitro characterization of goat skeletal muscle satellite cells – MS, MB (Animal production)

Seminar 12 – 11/01/22: Coffea arabica L. Resistant to Coffee Berry Borer (Hypothenemus hampei) Mediated by Expression of the Bacillus thuringiensis Cry10Aa Protein – MA, SQ, ZM (Plant production)

Seminar 13 – 18/01/22: Mass spider silk production through targeted gene replacement in Bombyx mori – AA, AD (Animal production)

* No self-evaluation

* No peer-evaluation

* Absence

* No presentation

Participants:

Mohammad Alzuwayed-MZ (classic micro – current plant).****/

Ahmad Groof-AG (classic animal – current micro).**/

Mohamed Abdellatif-ML (classic plant – current animal).*/

Kawthar Akbar-KA (classic micro – current plant).*/

Zahraa Almomen-ZM (classic animal – current micro).*/

Anfal Mahdi-AM (classic plant – current animal).*/

Suggested biotechnology papers (Thanks to Dr. Nasmah Bastaki):

Microbial papers:

1. Lawyer F.C., Stoffel S., Saiki R.K., Chang S.Y., Landre P.A., Abramson R.D. & Gelfand D.H. (1993) High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity. Genome Research 2, 275-87.

Plant papers:

1. Guo M., Ye J., Gao D., Xu N. & Yang J. (2019) Agrobacterium-mediated horizontal gene transfer: Mechanism, biotechnological application, potential risk and forestalling strategy. Biotechnology Advances 37, 259-70. (Review)
2. Indurker S., Misra H.S. & Eapen S. (2007) Genetic transformation of chickpea (Cicer arietinum L.) with insecticidal crystal protein gene using particle gun bombardment. Plant Cell Reports 26, 755-63.
3. Clough S.J. & Bent A.F. (1998) Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thaliana. The Plant Journal 16, 735-43.
4. Beyer P., Al-Babili S., Ye X., Lucca P., Schaub P., Welsch R. & Potrykus I. (2002) Golden Rice: Introducing the β-Carotene Biosynthesis Pathway into Rice Endosperm by Genetic Engineering to Defeat Vitamin A Deficiency. The Journal of Nutrition 132, 506S-10S.
5. Paine J.A., Shipton C.A., Chaggar S., Howells R.M., Kennedy M.J., Vernon G., Wright S.Y., Hinchliffe E., Adams J.L., Silverstone A.L. & Drake R. (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nature Biotechnology 23, 482-7.
6. Puchta H. (2003) Marker-free transgenic plants. Plant Cell, Tissue and Organ Culture 74, 123-34. (Review)
7. Ozyigit I.I. & Yucebilgili Kurtoglu K. (2020) Particle bombardment technology and its applications in plants. Molecular Biology Reports 47, 9831-47. (Review)
8. Tacket C.O. (2005) Plant-derived vaccines against diarrheal diseases. Vaccine 23, 1866-9. (Review)
9. Kusaba M. (2004) RNA interference in crop plants. Current Opinion in Biotechnology 15, 139-43. (Review)
10. Arshad W., Haq I.-u., Waheed M.T., Mysore K.S. & Mirza B. (2014) Agrobacterium-Mediated Transformation of Tomato with rolB Gene Results in Enhancement of Fruit Quality and Foliar Resistance against Fungal Pathogens. PLOS ONE 9, e96979.

Animal papers:

1.  Baldassarre H., Hockley D.K., Olaniyan B., Brochu E., Zhao X., Mustafa A. & Bordignon V. (2008) Milk composition studies in transgenic goats expressing recombinant human butyrylcholinesterase in the mammary gland. Transgenic Research 17, 863-72.
2. Cheng G., Fu C., Wang H., Adoligbe C., Wei S., Li S., Jiang B., Wang H. & Zan L. (2015) Production of transgenic beef cattle rich in n-3 PUFAs by somatic cell nuclear transfer. Biotechnology Letters 37, 1565-71.
3. Lee S.H., Gupta M.K., Ho Y.T., Kim T. & Lee H.T. (2013) Transgenic chickens expressing human urokinase-type plasminogen activator.  Poultry Science 92, 2396-403.
4. Snyder B.R. & Chan A.W.S. (2018) Progress in developing transgenic monkey model for Huntington’s disease. Journal of Neural Transmission 125, 401-17.
5. Hu S., Ni W., Sai W., Zi H., Qiao J., Wang P., Sheng J. & Chen C. (2013) Knockdown of Myostatin Expression by RNAi Enhances Muscle Growth in Transgenic Sheep. PLOS ONE 8, e58521.
6. Wen H., Lan X., Zhang Y., Zhao T., Wang Y., Kajiura Z. & Nakagaki M. (2010) Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons. Molecular Biology Reports 37, 1815-21.
7. Pawar N., Gireesh-Babu P., Sabnis S., Rasal K., Murthy R., Zaidi S.G.S., Sivasubbu S. & Chaudhari A. (2016) Development of a fluorescent transgenic zebrafish biosensor for sensing aquatic heavy metal pollution. Transgenic Research 25, 617-27.

Your presentation should include:

1. Title, student name, course, presentation date-slide.
2. Paper front page and abstract-slide.
3. General info about the organism-slide(s).
4. General info about the problem/goal of the study-slide(s).
5. General description about the methods used-silde(s).
6. Result (each figure or table in a separate slide).
7. Discussion slide about the usefulness of the study.
8. A final slide about your ideas/suggestions/ethical issues for implementing such studies in Kuwait.

To Do:

1.  Search for a classical biotechnology research paper and send a pdf to your instructor for approval.
2. Make a powerpoint file named (YourName_501).
3. Send a copy of your presentation a day before your actual presentation.
4. Non-presenters should read the paper and prepare to engage in discussion.
5. We will repeat the process with more recent publications.

Topics:

Seminar 1 – 06/04/21: General introduction and course planning – Hasan Alhaddad

Seminar 2 – 13/04/21: A bacterial clone synthesizing proinsulin – MZ (classic micro), Presentation: not presented MAlzuwayed1_501

Seminar 3 – 20/04/21: Expression of Naphthalene Oxidation Genes in Escherichia coli Results in the Biosynthesis of Indigo – KA (classic micro), Presentation: KawtherAkbar1_501

Seminar 4 – 27/04/21: Viable offspring derived from fetal and adult mammalian cells – AG (classic animal), Presentation: Ahmad1_510

Seminar 5 – 04/05/21: In vitro embryo production in camel (Camelus dromedarius) from in vitro matured oocytes fertilized with epididymal spermatozoa stored at 4 ◦C – ZM (classic animal), Presentation: Zahraa1_510

Seminar 6 – 11/05/21: The transformation of Zea maysseedlings with Agrobacterium tumefaciens – ML (classic plant), Presentation: ML1_510

Seminar 7 – 18/05/21: Transgenic tomato lines expressing human lactoferrin show increased resistance to bacterial and fungal pathogens – AM (classic plant), Presentation: Anfal1_501

Seminar 8 – 25/05/21: Design and synthesis of a minimal bacterial genome – AG (current micro), Presentation: Ahmad2_510

Seminar 9 – 01/06/21: Improving T-DNA Transfer to Tamarix hispida by Adding Chemical Compounds During Agrobacterium tumefaciens Culture – ZM (current micro), Presentation: Zahraa2_510

Seminar 10 – 08/06/21: Efficient Generation of Myostatin Gene Mutated Rabbit by CRISPR/Cas9 – ML (current animal), Presentation: ML2_510

Seminar 11 – 15/06/21: The effects of PEGylation on LNP based mRNA delivery to the eye – AM (current animal), Presentation: Anfal2_501

Seminar 12 – 22/06/21: A Nanobionic Light-Emitting Plant – MZ (current plant), Presentation: MZ2_510

Seminar 13 – 29/06/21: Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing – KA (current plant), Presentation: Kawther2_501