Research Topics & Seminar: Spring 2021

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* No self-evaluation
* No peer-evaluation
* Absence
* No presentation
Participants:
Mohammad Alzuwayed-MZ (classic micro – current plant).****/
Ahmad Groof-AG (classic animal – current micro).**/
Mohamed Abdellatif-ML (classic plant – current animal).*/
Kawthar Akbar-KA (classic micro – current plant).*/
Zahraa Almomen-ZM (classic animal – current micro).*/
Anfal Mahdi-AM (classic plant – current animal).*/
 
Suggested biotechnology papers (Thanks to Dr. Nasmah Bastaki):

Microbial papers:

  1. Lawyer F.C., Stoffel S., Saiki R.K., Chang S.Y., Landre P.A., Abramson R.D. & Gelfand D.H. (1993) High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity. Genome Research 2, 275-87.

Plant papers:

  1. Guo M., Ye J., Gao D., Xu N. & Yang J. (2019) Agrobacterium-mediated horizontal gene transfer: Mechanism, biotechnological application, potential risk and forestalling strategy. Biotechnology Advances 37, 259-70. (Review)
  2. Indurker S., Misra H.S. & Eapen S. (2007) Genetic transformation of chickpea (Cicer arietinum L.) with insecticidal crystal protein gene using particle gun bombardment. Plant Cell Reports 26, 755-63.
  3. Clough S.J. & Bent A.F. (1998) Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thaliana. The Plant Journal 16, 735-43.
  4. Beyer P., Al-Babili S., Ye X., Lucca P., Schaub P., Welsch R. & Potrykus I. (2002) Golden Rice: Introducing the β-Carotene Biosynthesis Pathway into Rice Endosperm by Genetic Engineering to Defeat Vitamin A Deficiency. The Journal of Nutrition 132, 506S-10S.
  5. Paine J.A., Shipton C.A., Chaggar S., Howells R.M., Kennedy M.J., Vernon G., Wright S.Y., Hinchliffe E., Adams J.L., Silverstone A.L. & Drake R. (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nature Biotechnology 23, 482-7.
  6. Puchta H. (2003) Marker-free transgenic plants. Plant Cell, Tissue and Organ Culture 74, 123-34. (Review)
  7. Ozyigit I.I. & Yucebilgili Kurtoglu K. (2020) Particle bombardment technology and its applications in plants. Molecular Biology Reports 47, 9831-47. (Review)
  8. Tacket C.O. (2005) Plant-derived vaccines against diarrheal diseases. Vaccine 23, 1866-9. (Review)
  9. Kusaba M. (2004) RNA interference in crop plants. Current Opinion in Biotechnology 15, 139-43. (Review)
  10. Arshad W., Haq I.-u., Waheed M.T., Mysore K.S. & Mirza B. (2014) Agrobacterium-Mediated Transformation of Tomato with rolB Gene Results in Enhancement of Fruit Quality and Foliar Resistance against Fungal Pathogens. PLOS ONE 9, e96979.

Animal papers:

  1.  Baldassarre H., Hockley D.K., Olaniyan B., Brochu E., Zhao X., Mustafa A. & Bordignon V. (2008) Milk composition studies in transgenic goats expressing recombinant human butyrylcholinesterase in the mammary gland. Transgenic Research 17, 863-72.
  2. Cheng G., Fu C., Wang H., Adoligbe C., Wei S., Li S., Jiang B., Wang H. & Zan L. (2015) Production of transgenic beef cattle rich in n-3 PUFAs by somatic cell nuclear transfer. Biotechnology Letters 37, 1565-71.
  3. Lee S.H., Gupta M.K., Ho Y.T., Kim T. & Lee H.T. (2013) Transgenic chickens expressing human urokinase-type plasminogen activator.  Poultry Science 92, 2396-403.
  4. Snyder B.R. & Chan A.W.S. (2018) Progress in developing transgenic monkey model for Huntington’s disease. Journal of Neural Transmission 125, 401-17.
  5. Hu S., Ni W., Sai W., Zi H., Qiao J., Wang P., Sheng J. & Chen C. (2013) Knockdown of Myostatin Expression by RNAi Enhances Muscle Growth in Transgenic Sheep. PLOS ONE 8, e58521.
  6. Wen H., Lan X., Zhang Y., Zhao T., Wang Y., Kajiura Z. & Nakagaki M. (2010) Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons. Molecular Biology Reports 37, 1815-21.
  7. Pawar N., Gireesh-Babu P., Sabnis S., Rasal K., Murthy R., Zaidi S.G.S., Sivasubbu S. & Chaudhari A. (2016) Development of a fluorescent transgenic zebrafish biosensor for sensing aquatic heavy metal pollution. Transgenic Research 25, 617-27.
Your presentation should include:
  1. Title, student name, course, presentation date-slide.
  2. Paper front page and abstract-slide.
  3. General info about the organism-slide(s).
  4. General info about the problem/goal of the study-slide(s).
  5. General description about the methods used-silde(s).
  6. Result (each figure or table in a separate slide).
  7. Discussion slide about the usefulness of the study.
  8. A final slide about your ideas/suggestions/ethical issues for implementing such studies in Kuwait.
To Do:
  1.  Search for a classical biotechnology research paper and send a pdf to your instructor for approval.
  2. Make a powerpoint file named (YourName_501).
  3. Send a copy of your presentation a day before your actual presentation.
  4. Non-presenters should read the paper and prepare to engage in discussion.
  5. We will repeat the process with more recent publications.
Topics:
Seminar 1 – 06/04/21: General introduction and course planning – Hasan Alhaddad
Seminar 2 – 13/04/21: A bacterial clone synthesizing proinsulin – MZ (classic micro), Presentation: not presented MAlzuwayed1_501
Seminar 3 – 20/04/21: Expression of Naphthalene Oxidation Genes in Escherichia coli Results in the Biosynthesis of Indigo – KA (classic micro), Presentation: KawtherAkbar1_501
Seminar 4 – 27/04/21: Viable offspring derived from fetal and adult mammalian cells – AG (classic animal), Presentation: Ahmad1_510
Seminar 5 – 04/05/21: In vitro embryo production in camel (Camelus dromedarius) from in vitro matured oocytes fertilized with epididymal spermatozoa stored at 4 ◦C – ZM (classic animal), Presentation: Zahraa1_510
Seminar 6 – 11/05/21: The transformation of Zea maysseedlings with Agrobacterium tumefaciens – ML (classic plant), Presentation: ML1_510
Seminar 7 – 18/05/21: Transgenic tomato lines expressing human lactoferrin show increased resistance to bacterial and fungal pathogens – AM (classic plant), Presentation: Anfal1_501
Seminar 8 – 25/05/21: Design and synthesis of a minimal bacterial genome – AG (current micro), Presentation: Ahmad2_510
Seminar 9 – 01/06/21: Improving T-DNA Transfer to Tamarix hispida by Adding Chemical Compounds During Agrobacterium tumefaciens Culture – ZM (current micro), Presentation: Zahraa2_510
Seminar 10 – 08/06/21: Efficient Generation of Myostatin Gene Mutated Rabbit by CRISPR/Cas9 – ML (current animal), Presentation: ML2_510
Seminar 11 – 15/06/21: The effects of PEGylation on LNP based mRNA delivery to the eye – AM (current animal), Presentation: Anfal2_501
Seminar 12 – 22/06/21: A Nanobionic Light-Emitting Plant – MZ (current plant), Presentation: MZ2_510
Seminar 13 – 29/06/21: Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing – KA (current plant), Presentation: Kawther2_501